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Journal of Applied and Natural Science
An International Journal | Print ISSN: 0974-9411 | Online ISSN: 2231-5209
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Abstract
Journal of Applied and Natural Science 8 (1): 416-422 (2016)
Isolation of endophytic actinomycetes from Syzygium cumini and their antimicrobial activity against human pathogens
Preeti Saini1*, Madhurama Gangwar1, Anu Kalia2, Narinder Singh3 and Deepti Narang4
1Department of Microbiology, Punjab Agricultural University, Ludhiana-141004 (Punjab), INDIA
2Electron Microscopy and Nanoscience Laboratory, Punjab Agricultural University, Ludhiana-141004 (Punjab), INDIA
3Department of Plant Pathology, Punjab Agriculural University, Ludhiana-141004 (Punjab), INDIA
4Department of Microbiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, (Punjab), INDIA
*Corresponding author. E-mail: saini.preeti7777@gmail.com
Abstract : Isolation of endophytic actinomycetes is an important step to screen antimicrobial compounds to curb the threat of drug-resistant strains of human pathogens. Out of the 50 endophytic actinomycetes obtained from surface sterilized root, stem and leaf tissues of Syzygium cumini, 50 isolates (30%) exhibited antimicrobial activity. Antistaphylococcal activity was displayed by most of the isolates, with maximum percent inhibition by J -10 (Mean of Inhibition Factor=12.12 mm2). A total of 8 isolates (4 each) were able to hydrolyse protein (proteinase activity) and solubilize chitin (chitinase activity). Results of thin layer chromatography confirm the production of chloramphenicol family |antibiotic by the isolate J-5. This is the first report providing an insight into untapped endophytic actinomycete milieu of Syzygium cumini yet to be explored which might be a promising source for novel antimicrobial agents.

Keywords :Antagonism, Chitinase, Protease, SEM, Syzygium cumini, TLC
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