Applied and Natural Science Foundation
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Journal of Applied and Natural Science
An International Journal | Print ISSN: 0974-9411 | Online ISSN: 2231-5209
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Journal of Applied and Natural Science 8 (1): 412-415 (2016)
Effect of phytohormones on shoot apex and leaf explants of Withania somnifera (Ashwagandha)
Archana Rani*, M. Kumar and Sanjeev Kumar1
Department of Agricultural Biotechnology and Molecular Biology, (FBS & H), Rajendra Agricultural University, Pusa-848125 (Bihar), INDIA
1Department of Plant Pathology, College of Agriculture, Jawaharlal Nehru Krishi Vishwa Vidayalaya Jabalpur- 482004 (M.P.), INDIA
*Corresponding author. E-mail:
Abstract : An efficient protocol for callus induction of Withania somnifera through in vitro culture of shoot apex and leaf explant was standardized. Of the various combinations of phytohormones evaluated, MS media supplemented with 6-furfuryl aminopurine (KIN) 0.5 mg/l + 2,4-dichlorophenoxy acetic acid (2, 4-D) 2.0 mg/l was found to be best for mean callus induction (86%) in leaf explants after 6 weeks of culture and in case of shoot apex expant the best response and growth of callusing was observed on MS medium supplemented with 2,4-D 1.0 mg/l + BAP 2.0 mg/l (77%).The response of callus growth increases gradually with the reductions in concentration of KIN in culture medium of both the explants. This protocol might be used in further research for mass propagation of W. somnifera via indirect regeneration methods.

Keywords : Callus induction, Leaf and shoot apex explants, Withania somnifera explant
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