Applied and Natural Science Foundation
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Journal of Applied and Natural Science
An International Journal | Print ISSN: 0974-9411 | Online ISSN: 2231-5209
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Journal of Applied and Natural Science 8 (1): 405-411 (2016)
Process optimization of supercritical carbon dioxide (SC-CO2) extraction parameters for extraction of deoxynojirimycin (1-DNJ) from mulberry (Morus alba L.) leaves
V. Ramya1, Udaykumar Nidoni1*, Sharangouda Hiregoudar1, C. T. Ramachandra1, J. Ashoka2 and V. Lavanya 1
1Department. of Processing and Food Engineering, College of Agricultural Engineering, University of Agricultural Sciences, Raichur (Karnataka), INDIA.
2Department of Entomology, College of Agriculture, University of Agricultural Sciences, Raichur (Karnataka), INDIA.
*Corresponding author. E-mail:
Abstract : In the present study, supercritical fluid extraction (SFE) technology was applied to extract deoxynojirimycin (1 -DNJ) from mulberry leaf powder using carbon dioxide (CO2) as major extraction solvent with ethanol as cosolvent, and extraction parameters such as pressure (100, 150 and 200 bar), temperature (40, 50 and 60 °C) and dynamic extraction time (40, 60 and 80 min) were systematically investigated by full factorial design to obtain the optimum extraction efficiency and extraction yield. Under optimized conditions (pressure of 200 bar, temperature of 50 °C and dynamic extraction time of 80 min), DNJ enriched extract was obtained with high extraction efficiency (96.46 %) and extraction yield (13.41 %), enabling this product to use for nutraceutical purpose. The results indicated that SC-CO2 extraction is a promising and alternative process for recovering the bioactive compounds from mulberry leaves.

Keywords :Carbon dioxide, Deoxynojirimycin, Extraction efficiency, Extraction yield, Mulberry, Supercritical fluid extraction
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