Journal of Applied and Natural Science 8 (1) : 225-231 (2016)
Moisture stress induced changes in metabolites and cellular functions in
chickpea (Cicer arietinum L.) genotypes
Navkiran Randhawa1*, Jagmeet Kaur1, Satvir Kaur2 and Sarvjeet Singh1
1Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana- 141004 (Punjab), INDIA
2Department of Biochemistry, Punjab Agricultural University, Ludhiana- 141004 (Punjab), INDIA
*Correrspnding author. E mail:firstname.lastname@example.org
Abstract : The present investigation was aimed to study influence of moisture stress in in vitro identified tolerant
(GL28151, RSG963, PDG3) and sensitive (GL22044, GNG1861, PBG1) chickpea genotypes under field conditions.
Moisture stress treatments included crop sown with one pre-sowing irrigation (WSVFP), irrigation withheld at flower
initiation stage (WSF), irrigation withheld at pod initiation stage (WSP) and control (irrigated as and when required).
Osmolytes (in seeds) viz. total soluble sugars, starch, proline, cellular functions; relative water content, membrane
permeability index and lipid peroxidation (in leaves), antioxidant enzymes (at pod filling stage) viz. peroxidase, catalase, superoxide dismutase, glutathione reductase were estimated in chickpea seeds under control and stressed
conditions. WSVFP was most severely affected by moisture stress followed by WSP and WSF and emphasized on pod
intuition stage as critical stage attributable to hindered transport of assimilates towards formation of pods and development of seeds under stress imposed by lack of sufficient moisture. Highest accumulation of total soluble sugars
(73.33), starch (73.12), proline (2.04) in mg/g fresh weight, least percentage reduction over control in relative water
content (20.3), membrane permeability index (18.8) and minimal lipid peroxidation (31.3) accompanied by significantly enhanced activities of antioxidant enzymes under WSVFP rendered moisture stress tolerance in RSG963. The
pronounced cellular damage, lesser alleviation in the content of osmolytes, antioxidant enzymes activity was observed in sensitive genotype GL22044 under stress treatments. High molecular weight protein bands were found
either absent or of low intensity in sensitive genotypes (GL22044, GNG1861 and PBG1) under severe stress treatment (WSVFP).
Keywords : Biochemicals, Cellular Chickpea, Cicer arietinum, Moisture stress