Applied and Natural Science Foundation
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Journal of Applied and Natural Science
An International Journal | Print ISSN: 0974-9411 | Online ISSN: 2231-5209
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Abstract
Journal of Applied and Natural Science 8 (1) : 128-132 (2016)
A competent protocol for large scale production of sugarcane (Saccharum officinarumL.) through meristem culture
J. Udhutha1, S. C. Mali1 and H. A. Sahare2*
1Main Sugarcane Research Station, Navsari Agriculture University, Navsari- 396 450(Gujarat), INDIA
2Department of floriculture and Landscape Architecture, Navsari Agriculture Univeristy, Navsari- 396 450 (Gujarat), INDIA
* Corresponding author. Email: - homrajsahare82@gmail.com
Abstract : A rapid micro propagation and acclimatization response of two different varieties of sugarcane Co86032 and CoN 04131(Saccharum officinarum L.) was obtained in this study. The shoot apical meristem of different sizes wascultured on Murashige and Skoog medium supplemented with different concentrations and combinations of benzylaminopurine and kinetin either alone or in combination with each other alongwith GA3. Best shoot formation response in Co 86032 was obtained on MS medium containing 1.5mg/l BAP while in CoN 04131 the combination of 0.5 mg/l BAP with 0.25 mg/l Kinetin showed best shoot formation response from apical meristem. Meristem of 3.0 mm size proved to be the best size for micropropagation of sugarcane. Excellent multiplication response of In vitro formed shoots was obtained when the concentration of BAP was decreased to 1.0 mg/l in Co 86032and 0.25 mg/l BAP and Kin in CoN 04131 (i.e. 0.25 mg/lBAP + 0.25 mg/l Kinetin. MS medium containing 1.0 mg/l NAA and 2.0 mg/ l IBA showed 100% rooting response of In vitro regenerated shoots of both the varieties of sugarcane within eight days of inoculation. Best hardening response was obtained in sand+ soil + pressmud (1:1:1) media.

Keywords : Benzylaminopurine, Gibberellin, Kinetin, Meristem culture and Naphthalene acetic acid
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