Applied and Natural Science Foundation
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Journal of Applied and Natural Science
An International Journal | Print ISSN: 0974-9411 | Online ISSN: 2231-5209
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Journal of Applied and Natural Science 8 (1): 93-96 (2016)
Phosphate solubilizing activity of Pseudomonas fluorescens PSM1 isolated from wheat rhizosphere
Anurag Yadav1*, Kusum Yadav2, Anupam Vashistha3
1Department of Microbiology, College of Basic Science & Humanities, S.D. Agricultural University, S.K. Nagar (Gujarat), INDIA
2Department of Biochemistry, University of Lucknow, Lucknow (Uttar Pradesh), INDIA
3Department of Microbiology, Division of Life Sciences, S.B.S. (PG) Institute of Biomedical Sciences & Research, Balawala, Dehradun (Uttarakhand), INDIA
*Corresponding author. E-mail:
Abstract :A study was designed to screen and analyze the efficient phosphate solubilizing bacteria (PSBs) from wheat rhizosphere. Five biovars of Pseudomonas fluorescens (PSM1, PSM2, PSM3, PSM4 and PSM5) were isolated from wheat rhizosphere and Bacillus megaterium MTCC 8755 procured from microbial type culture collection (MTCC) Chandigarh, India. The P. fluorescens biovar PSM1 was observed to be most efficient phosphate solubilizer. Inoculation of P. fluorescens PSM1 and B. megaterium MTCC 8755, alone and in combination, caused the highest phosphate solubilization at pH 5. At this pH, maximum phosphate solubilization was observed with B. megaterium MTCC 8755 inoculation (8.2 mg mL-1) on sixth day of incubation with P. fluorescens PSM1 (8 mg mL-1) on seventh day of incubation and with dual bacterial treatment (10.5 mg mL-1) on the fourth day of incubation. A correlation coefficient of linear regression equation of phosphate solubilization with pH indicated that pH value of the medium was directly correlated with tricalcium phosphate solubilization. The study will help in choosing soil pH specific PSB inoculant for optimizing plant growth.

Keywords :Phosphate solubilizing bacteria, Pseudomons fluorescens, Rhizosphere
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