Applied and Natural Science Foundation
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Journal of Applied and Natural Science
An International Journal | Print ISSN: 0974-9411 | Online ISSN: 2231-5209
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Journal of Applied and Natural Science 7 (1): 489–492 (2015)
Inhibitory effect of bacterial antagonists on the growth of Macrophomina phaseolina (Tassi.) Goid. Causing charcoal rot of sunflower (Helianthus annus L.) invitro
A. Karmel Reetha*1 and S. Mohan2
1Department of plant pathology, Vanavarayar Institute of Agriculture, Pollachi - 642103 Tamil Nadu, INDIA
2Department of plant Pathology, Agricultural Collège and Research Institute, Madurai - 625104 Tamil Nadu,INDIA
*Corresponding author. E-mail:
Abstract : Charcoal rot caused by Macrophominaphaseolinais a major disease causing in sunflower plant. The pathogen invasion occurs from the seedling to maturity stage. To overcome this problem in vitro, sensitivity of M. phaseolina was determined through inhibition zone technique to various isolates of antagonistic bacteria like seven isolates of Pseudomonasfluorescens (EPf2, EDPf3, APf4, CPf5, MPf6, KPf7andPf1)and seven isolates Bacillus subtilis (EBs1, EDBs2, ABs3, CBs4, MBs5, KBs6andBs10) amended into PDA medium. The results showed that the entire bacterial antagonist were effective against the fungus M. phaseolina and exhibited appreciable amount of inhibition. Among these bacterial antagonistsignificantly compared to the control P.fluorescens (Pf1) proved to be the most effective (71.49 %) with an inhibition zone of 5.00 mm reducing the colony growth of M. Phaseolina followed B. subtilis (65.92 %)inhibition zone of 17.80 mm respectively over control. However, from these studies it is concluded that an isolate of various antagonist can vary in its sclerotia producing ability on root.

Keywords :Bacterial biocontrol, In-vitro, Macrophomina phaseolina, Sunflower
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