Applied and Natural Science Foundation
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Journal of Applied and Natural Science
An International Journal | Print ISSN: 0974-9411 | Online ISSN: 2231-5209
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Abstract
Journal of Applied and Natural Science 7 (1): 477–481 (2015)
Prevalence, serodiversity and antibiogram of enterotoxigenic Escherichia coli (ETEC) in diarrhoeic calves and lambs of Kashmir valley (J&K), India
Rusheeba Manzoor1, Mohammad Irfan Shah2, Asma-ul-husna1, Shakil Ahmad Wani1, Farhat Pandit1, Pervaiz Ahmad Dar1, Mohammad Iqbal Mir3
1Division of Veterinary Microbiology and Immunology, Faculty of Veterinary Sciences and Animal Husbandry Sher-e-Kashmir University of Agricultural Sciences & Technology, Kashmir Shuhama Campus, Srinagar- 190006, INDIA
2Division of Veterinary Pharmacology and Toxicology, IVRI, Izatnagar, Bareilly 243122, Uttar Pradesh
3Division of Fish Pathology, Faculty of Fisheries Sher-e-Kashmir University of Agricultural Sciences & Technology, Kashmir, Rangil, Ganderbal 190006
*Corresponding author. E-mail: qadrijavid2008@gmail.com
Abstract : Enterotoxigenic E. coli (ETEC) is the major cause of diarrhoea in neonatal animals. This study determined the prevalence, serological diversity, virulence gene profile and in-vitro antibiogram of ETEC isolates from diarrhoeic faeces of calves and lambs. The prevalence rate of ETEC in lambs was recorded 18.46 % with O8 as predominant serotype. However, in calves the prevalence rate was recorded 8.57 % with O15 and O26 as predominant serotypes. The antibiogram screening showed differential susceptibility pattern among ETEC isolates with highest resistance to ampicillin and highest sensitivity to enrofloxacin. In the present study, for the first time it was reported that the diarrhoea in calves and lambs occur due to virulent gene est not due to elt gene, which was absent in all the isolates.

Keywords : Calves, Diarrhoea, Lambs, PCR
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