Journal of Applied and Natural Science 7 (1): 131–137 (2015)
Regeneration of Indian maize genotypes (Zea mays L.) from immature embryo culture through callus induction.
N. Malini*1, C. R. Ananadakumar2 and S. Hari ramakrishnan3
1ARS, Kovilpatti, Tamil Nadu Agricultural University, Coimbatore, INDIA.
2Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, INDIA.
3Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, INDIA.
*Corresponding author. E-mail: email@example.com
Abstract : Callus induction and regeneration ability of five maize genotypes UMI 757, UMI 615,
UMI 112, UMI 285 and CO 1 and one promising maize hybrids COH(M) 5 were investigated using 14 days old immature
embryos as explants. Callus induction depends on genotype, explants (age and size of explants), medium, type of auxin
and their concentration. Explants grown on Murashige and Skoog (MS) medium supplemented with 1.5 mg/l 2, 4 - D
(2,4-dichloro phenoxy acetic acid), 0.3 mg/l kinetin with 30 g/l maltose showed the highest percentage of embryogenic
callus induction. Among the six genotypes tested, COH (M) 5 maize hybrids have highest percentage of embryogenic calli.
The embryogenic calli incubated on MS medium supplemented with 1.5 mg/l BAP (Benzyl Amino Purine), 0.2 mg/l NAA
(Naphthalene Acetic Acid) with 1.0 mg/l kinetin was found to give the highest organogenesis response and regeneration of plantlets.
Keywords :Callus induction, Immature embryo, Maize, Regeneration