Journal of Applied and Natural Science 6 (2): 852-856 (2014)
Greenhouse evaluation of Beauveria bassiana (Balsamo) Vuillemin against
Aphis craccivora (Das) on Fenugreek.
K. Selvaraj* and H. D. Kaushik
Department of Entomology, CCS Haryana Agricultural University, Hisar-125004, INDIA
*Division of Crop Protection, Central Research Institute for Jute and Allied Fibres, Kolkata-700120, INDIA
*Corresponding author. E-mail: email@example.com
Abstract : The bioassay studies were carried out to determine the LC50 and LT50 of Beauveria bassiana against
Aphis craccivora on fenugreek under greenhouse conditions. The results revealed that, the cumulative corrected
mortality (CCM) was 43.50% at higher concentration (1×1010 spores/ml) and it was 20.85% at lowest concentration
(1×104 spores/ml) at one day after treatment (DAT). The CCM decreased with decreasing conidial spore concentration.
Likewise, at 2, 3, 4, 5, 6 and 7 DAT, almost same trend was observed. At 7 DAT, the CCM was 85.04% and
55.21% at 1×1010 spores/ml and 1×104 spores/ml, respectively. The LC50 value of B. bassiana against A. craccivora
was 1.2×108 spores/ml. Mean lethal time (LT50) values were worked out 73, 89, 97, 112, 126, 138 and 157 hours for
1010, 109, 108, 107, 106, 105 and 104 spores/ml, respectively. By testing the field efficacy of B. bassiana against A.
craccivora, this insect pathogenic fungus can be used as potential biocontrol agent for the sustainable management
of aphid in fenugreek crop.
Keywords : Aphis craccivora, Beauveria bassiana, LC50, LT50 value, Pathogenicity.