Journal of Applied and Natural Science 6 (2): 366-370 (2014)
Optimization of fermentation conditions for producing Indian rock bee (Apis
dorsata) mead using response surface methodology.
N. Srimeena*, S. Gunasekaran and R. Murugesan
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore - 641003 (Tamil Nadu),
*Corresponding author. E-mail: firstname.lastname@example.org
Abstract : Mead is a traditional drink which results from the alcoholic fermentation of diluted honey carried out by
yeast (Saccharomyces cerevisiae KF233529). The present investigation was carried out for the optimization of
fermentation parameters for maximizing the yield of ethanol. Response Surface Methodology (RSM) based central
composite design was employed to obtain best combination of temperature, fermentation time and total soluble
solids (TSS). The optimum conditions for ethanol yield were temperature 28°C, TSS 15°Brix and 6 days after
fermentation. The model showed that the value of R2 (0.9998) was high and p- value of interaction of variance was
<0.0001. Hence the model can be said to be of highly significant.
Keywords :Ethanol, Fermentation time, Mead, Response Surface Methodology (RSM), Temperature, TSS.