Applied and Natural Science Foundation
Its publication:
Journal of Applied and Natural Science
An International Journal | Print ISSN: 0974-9411 | Online ISSN: 2231-5209
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Journal of Applied and Natural Science 6 (2): 366-370 (2014)
Optimization of fermentation conditions for producing Indian rock bee (Apis dorsata) mead using response surface methodology.
N. Srimeena*, S. Gunasekaran and R. Murugesan
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore - 641003 (Tamil Nadu), INDIA
*Corresponding author. E-mail:
Abstract : Mead is a traditional drink which results from the alcoholic fermentation of diluted honey carried out by yeast (Saccharomyces cerevisiae KF233529). The present investigation was carried out for the optimization of fermentation parameters for maximizing the yield of ethanol. Response Surface Methodology (RSM) based central composite design was employed to obtain best combination of temperature, fermentation time and total soluble solids (TSS). The optimum conditions for ethanol yield were temperature 28°C, TSS 15°Brix and 6 days after fermentation. The model showed that the value of R2 (0.9998) was high and p- value of interaction of variance was <0.0001. Hence the model can be said to be of highly significant.

Keywords :Ethanol, Fermentation time, Mead, Response Surface Methodology (RSM), Temperature, TSS.
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