Journal of Applied and Natural Science 6 (2): 748-750 (2014)
Enzyme changes during seed storage in groundnut (Arachis hypogaea L.).
M. Ameer Junaithal Begum1*, P. Balamurugan1, K. Vanagamudi1, K. Prabakar2 and R.
1Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore (Tamil Nadu), INDIA
2Department of Plant pathology, Tamil Nadu Agricultural University, Coimbatore (Tamil Nadu), INDIA
*Corresponding author. E-mail: firstname.lastname@example.org
Abstract : A change in enzyme activity in seeds due to ageing is a topic of scientific importance. Vigour is essentially
a physiological phenomenon influenced by the reserved metabolites, enzyme activities and growth regulators. The
exact cause of loss of seed vigour and viability is still unknown as deterioration of seed is a complex process. In the
presence of oxygen, ageing of seed can lead to peroxidative changes in polyunsaturated fatty acids. The free radical
-induced non-enzymatic peroxidation, which has the potential to damage membrane, is likely to be a primary cause
of deterioration of stored seeds. Certain anabolic enzymes help in maintaining viability while some catabolic
enzymes decrease viability. The seed catalase and peroxidase activity seem to be decreased during storage. The
results revealed that the peroxidase enzyme activity decreased from 0.236 to 0.444 OD 10 min-1 when storage
period increased. A decrease in catalase activity from 0.454 to 0.444 μg H2O2 mg-1 min-1 followed by a small
increase from 0.434 to 0.452 μg H2O2 mg-1 min-1 was observed during storage. But the activity of lipase enzyme
increased from 0.236 to 0.231 meq min-1g-1 of sample when the storage period was increased. The study would help
to know the deterioration pattern of stored groundnut seeds.
Keywords : Enzyme changes, Groundnut, Seed, Storage.