Applied and Natural Science Foundation
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Journal of Applied and Natural Science
An International Journal | Print ISSN: 0974-9411 | Online ISSN: 2231-5209
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Abstract
Journal of Applied and Natural Science 6 (2): 693-695 (2014)
Changes in protein profile and RNA content of Apis mellifera worker pupa on parasitization with Tropilaelaps clareae.
Jamuna Negi* and Neelima R. Kumar
Department of Zoology, Panjab University, Chandigarh-160014, INDIA
*Corresponding author. E-mail: james.negi@gmail.com
Abstract : Tropilaelaps infestation of Apis mellifera pupa caused reduction in total protein concentration of the infested pupa. This may be due to protein feeding by mite. The additional protein fractions observed by SDS-PAGE and the difference in the nature of free amino acids suggested synthesis of newer proteins perhaps related to the stress response of the host. This opinion is strengthened by the increase in concentration of RNA ( 184.580±36.987 in non-infested pupa and 293.402±50.329 in infested pups) observed in the present study which was responsible for increased transcription of genes encoding antiparasite peptides as reported by other workers..

Keywords : Apis mellifera, Peptides, Topilaelaps clareae.
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