Journal of Applied and Natural Science 6 (2): 649-653 (2014)
In vitro antioxidant and antimicrobial activity of carotenoid pigment
extracted from Sporobolomyces sp. isolated from natural source.
M. R. A. Manimala* and R. Murugesan
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore-641003, (Tamil Nadu),
*Corresponding author. E-mail: email@example.com
Abstract : The aim of the present study was to isolate and study
about the antioxidant and antibacterial activity of carotenoid pigment.
Sporobolomyces sp. isolated from the phyllosphere surface of rice plant has
found to produce carotenoid pigment. The present investigation was carried out
for antioxidant assays viz., DPPH, iron reducing and metal chelating
activity. A steady increase in the antioxidant activities was observed in the
carotenoid pigment with raising the pigment concentration. In the present study,
the maximum antioxidation characteristics of carotenoid by DPPH, iron reducing
and metal chelating assays (75.04 %, 1.88 % and 59.32 %) were achieved by
pigmentation of Sporobolomyces sp. at the concentration of 100 μg ml-1. The
antibacterial activity was studied on several organisms like Enterococcus
sp., Staphylococcus aureus, Streptococcus faecalis, Bacillus
subtilis, Escherichia coli and Pseudomonas aeruginosa. Among the
six pathogens, the pigment was found to be more effective against E. coli
(2.9 cm) and S. aureus (2.6 cm). This study revealed that yeast carotenoid
pigment was a potential source for its use in food and pharmaceutical
Keywords : Antimicrobial activity, Antioxidant activity, Carotenoid
pigment, Sporobolomyces sp.