Journal of Applied and Natural Science 6 (2): 451-456 (2014)
Cultural and morphological characterization of rhizospheric isolates of fungal
K. K. Sharma1* and U. S. Singh2strong>
1Vivekanand Parvatiya Krishi Anusandhan Sansthan, Almora-263601 (Uttarakhand), INDIA
2International Rice Research Institute - 9th Floor, Aggarwal Corporate Tower, Rajendra Place, New Delhi - 110 008, INDIA
*Corresponding author. E-mail: firstname.lastname@example.org
Abstract : The genus Trichoderma contains species that are of a great economic importance due to their ability to
act as biological control agents against a large variety of fungal plant pathogens. In the present investigation thirty
isolates of the Trichoderma sp. were obtained from the rhizosphere soils of different plants at different locations at
Nainital, Almora, Udham Singh Nagar, Derhadun, Haridwar and Tehri Garhwal districts of Uttarakhand (India). The
isolates were characterized on the basis of their cultural and morphological characteristics. The cultural characteristics
included linear growth, colony colour, pigmentation and growth pattern. Morphological characteristics studied were
structure, shape and arrangement of conidiophores, phialides and conidia. Out of thirty isolates, 6 isolates namely
PB10, PB13, PB23, PB26, PB27 and PB28 were identified as T. virens and remaining 24 isolates as T. harzianum.
Keywords : Characterization, Cultural, Morphological, Phialides, Trichoderma.