Journal of Applied and Natural Science 6 (2): 402-408 (2014)
Effect of nitrogen and sulphur nutrition on yield parameters and protein
composition in soybean [Glycine max (L.) Merrill].
A. Sharma* and S. Sharma
Department of Biochemistry, Punjab Agricultural University, Ludhiana-141004, INDIA
*Corresponding author. E-mail: firstname.lastname@example.org
Abstract : Accumulation patterns of various protein fractions in
seed, yield and protein quality parameters of soybean cultivar SL 525 harvested
at different stages of development from plants grown under different treatments
viz., nitrogen (urea @ 31.25 kg N ha-1), sulphur (gypsum @
20 kg S ha-1) and combined application of nitrogen (N) and sulphur
(S) were investigated. Application of S alone or combined with N significantly
increased plant height, number of seeds per pod and total seed yield.
Sulphur-containing amino acids viz., methionine and cysteine increased
significantly (P≤0.05) by all the treatments in comparison to control and
maximum increase of 1.3 fold was observed by urea application. The contents of
different protein fractions viz., albumin, globulin and glycinin (a
subfraction of globulin) increased under the effect of N and S applied alone or
in combination from 30 DAP to maturity with maximum accumulation at maturity as
compared to control (without N or S application). Gypsum @ 20 kg S ha-1
resulted in higher accumulation of glycinin (11S) relative to β-conglycinin (7S) fraction of globulin protein in soybean
seed thereby increasing 11S:7S ratio indicating improvement in soybean seed quality. The current study showed
that improvement of soybean nutritional quality can be achieved by application of gypsum @ 20 kg S ha-1 along with
recommended N doses under agroclimatic conditions of Punjab.
Keywords :Nitrogen, Protein quality, Soybean, Sulphur, Yield.