Journal of Applied and Natural Science 6 (1): 197-201 (2014)
Normalizing of estrous cycle in polycystic ovary syndrome (PCOS) induced
rats with Tephrosia purpurea (Linn.) Pers.
Akanksha P. Thakor* and Anuradha J. Patel1
Department of Biotechnology, N. B. Mehta Science College, Bordi-401701, INDIA
1Department of Zoology, Bhavans College, Andheri, Mumbai, INDIA
*Corresponding author. E-mail:email@example.com
Abstract : This study evaluates the potentiality of an herbal plant Tephrosia purpurea (Linn.) Pers. in the treatment
of Letrozole induced polycystic ovary syndrome (PCOS) using female Albino wistar rats. Seed powder of
T. purpurea at concentration of 200 mg/kg body weight with milk as a carrier vehicle 3ml/kg body weight, given for at
least 3 consecutive estrous cycles. Vaginal smear, FSH, LH, Testosterone, and Estrogen were analyzed to
determine the fluctuations in sex steroid level in PCOS induced rats. The plasma testosterone and estrogen level
were found to be significantly increased in rats with PCOS whereas Follicle stimulating hormone and Leutinizing
hormone did not show any changes. When compared with control the PCOS induced rats showed characteristic
ovary with high incidence of ovarian cyst. All the parameters assessed were significantly improved after the
treatment with T. purpurea and achieved level close to 80% normalcy. Effect of T. purpurea significantly reduced
histopathological changes in ovary and endocrinological and biochemical changes induced by hyperandrogenism.
Further to check ovulation and fertility female rats were mated and pregnancy was confirmed. Thus potential of
T. purpurea in the treatment of PCOS using an animal model suggested being a good alternative therapy in the
treatment of PCOS.
Keywords : Carboxymethylecellulose, Estrogen, Estrous cycle, Letrozole, Polycystic ovary syndrome, Testosterone,