Applied and Natural Science Foundation
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Journal of Applied and Natural Science
An International Journal | Print ISSN: 0974-9411 | Online ISSN: 2231-5209
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Journal of Applied and Natural Science 6 (1): 159-163 (2014)
In vitro propagation of Withania somnifera (L.) Dunal. from shoot apex explants
Archana Rani*, M. Kumar and Sanjeev Kumar1
Department of Agricultural Biotechnology and Molecular Biology, (FBS & H), Rajendra Agricultural University Pusa-848125 (Bihar), INDIA
1Department of Plant Pathology, College of Agriculture, Jawaharlal Nehru Krishi Vishwa Vidyalaya Jabalpur- 482004 (M.P.), INDIA
*Corresponding author. E-mail:
Abstract : In vitro rapid micropropagation of Withania somnifera; has been achieved using shoot apex explants, when cultured on MS medium with different auxins: Indole Butyric Acid (IBA) 0.5-4 mg/l, Indole Acetic Acid (IAA) 2 mg/l and Naphthalene Acetic Acid (NAA) 0.5-3 mg/l and cytokinin: Benzyl Amino Purine (BAP) 0.5-4 mg/l. NAA and BAP stimulated direct as well as callus mediated multiple shoot. IBA alone and in combination with IAA stimulated rhizogenesis, while IBA with NAA proliferated callus-mediated rhizogenesis. The rooted plantlets through sequential hardening process could be established in the field.

Keywords : Micropropagation, Shoot apex explant, Rhizogneesis, Withania somnifera
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