Applied and Natural Science Foundation
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Journal of Applied and Natural Science
An International Journal | Print ISSN: 0974-9411 | Online ISSN: 2231-5209
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Abstract
Journal of Applied and Natural Science 5 (2): 357-360 (2013)
Analysis of bioactive phytochemicals and evaluation of antioxidant activity of a medicinal plant, Boerhaavia diffusa L.
Deepti Malhotra, Fouzia Ishaq1, Amir Khan2* and A. K. Gupta3
Department of Biotechnology, Shri Guru Ram Rai (P.G.) College, Dehradun (U.K.), INDIA
1Department of Zoology and Environmental Science, Gurukula Kangri University, Haridwar-249404 (U.K.), INDIA
*2Glocal School of Life Science, The Glocal University (GU), Ali Akbarpur, Mirzapur Pol, Saharanpur - 247001 (U.P.), INDIA
3Department of Chemistry, Shri Guru Ram Rai (P.G.) College, Dehradun (U.K.), INDIA
*Corresponding author. E-mail: amir amu@gmail.com
Abstract : Boerhaavia diffusa L. (Family: Nyctaginaceae) commonly known as Punarnava is an herbaceous, spreading vine widely distributed in the tropical and subtropical regions in the world. The plants are a rich source of vitamins, minerals, protein and carbohydrate. The present study was carried out to determine the concentration of some bioactive phytochemicals (ascorbic acid, carotenoids, total phenolics, protein and carbohydrate) and their antioxidant activity in punarnava. Results showed the values for ascorbic acid (16.75±1.72 and 18.86±1.12 mg/100g of Fresh Weight), carotenoids (1.36±0.10 and 1.98±0.11 ìg/g of Fresh Weight), protein (122.975±6.27 and 134.45±6.23 mg/g of dry weight) and carbohydrate (56.67±5.77 and 60.11±5.23 mg/g of dry weight) for aqueous and methanolic of root extracts of B.diffusa respectively. Methanolic root extracts showed greater antioxidant activity than the aqueous extracts using DPPH method.

Keywords : Antioxidant property, Boerhaavia diffusa, DPPH, EC50, Flavonoid content.
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