Journal of Applied and Natural Science 5 (1): 41-46 (2013)
Culture and physiological variability in Rhizoctonia solani, responsible for foliar and lesions on aerial part of soybean
Lalan Sharma1*, Sanjay Goswami2 and D. T. Nagrale3
1Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar- 263145 (UK), INDIA
2Department of Plant Pathology, Punjab Agriculture University, Ludhiana- 141 004, INDIA
3Department of Plant Pathology and Agricultural Microbiology, Mahatma Phoole Agricultural University, Rahuri- Rahuri-413722, INDIA
*Corresponding author. E-mail: email@example.com
Abstract : Foliar blight of soybean is one of the major fungal diseases. Rhizoctonia solani isolated from soybean growing in tarai regions of Uttarakhand. Six isolates of R. solani has been characterized on the basis of cultural and physiological nature such as colony diameter, growth, colour and sclerotia formation were recorded. Potato Dextrose Agar (PDA) was found best for growth and development. Two isolates (Lakhimpur and Pantnagar) covered the whole plates (90 mm) in 48 hrs. However, maximum number of sclerotia and weight was recorded on Czapek Dox agar medium. Overall radial growth supporting is recorded Corn Meal Agar Medium. Varied range of temperatures i.e. 10, 15, 25, 30, 35 and 400C was tested and found better growth of different isolates of R. solani at 10 - 400C, with an optimum growth temperature at 300C. Isolates were grown on five broth media (Asthana & Hawkers, Potato Dextrose Agar, Czapek’s Dox Agar, Corn Meal Agar and Richards Agar) for fresh, dry weight and oat meal broth culture filtrates of all isolates was used in phytotoxic effects. It recorded that maximum fresh and dry weight was observed on corn meal agar medium. The maximum reduction in radical and plumule length of germinating seeds were recorded in Haldichaur isolate.
Keywords : Cultural, Foliar blight, Physiological, Phytotoxic, Rhizoctonia solani and Soybean.