Journal of Applied and Natural Science 5 (1): 142-147 (2013)
Evaluation of relationship between microbial load and drug efficacy of Andrographis paniculata during storage
G. Khandelwal1*, Aparajita Gupta1, Deepak Dwivedi2, G. Prasad1 and D.K. Maheshwari1
1Department of Botany and Microbiology, Gurukula Kangri Vishwavidyalaya, Haridwar - 249404 (Uttarakhand), INDIA
2Department of Microbiology, Barkatullah University, Bhopal (Madhya Pradesh), INDIA
*Corresponding author. E-mail: firstname.lastname@example.org
Abstract : Three different extracts of Andrographis paniculata obtained by using three different solvents i.e. acetone, ethanol and water, were used to find out correlation of antimicrobial potency of the drug among them and with storage period of six months. Antibacterial activities were assessed by well diffusion method against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Bacillus subtilis, and Salmonella typhi and antifungal activity by food poisoning technique against Candida albicans. No definite pattern of antimicrobial nature in acetone, ethanol and aqueous extract could be observed. Acetone extract showed maximum inhibitory (18.3mm) effect among all extract in general. Ethanol extract could attain second position and aqueous extract failed to inhibit growth of any organism even at 100% concentration. A declined trend of inhibitory effect of extract with increased number of storage days has been found showing a negative relation between inhibitory effect of the drug and storage duration. But positive correlation between inhibitory effect and concentration has been recorded. No inhibition was recorded against E. coli by any of the extract Bacterial load in term of CFU/g was found tremendously enhanced with increase of storage period. Negative correlation between microbial load and drug efficacy had been established while positive correlation between microbial load and storage period had been recorded.
Keywords : Andrographis paniculata, Antimicrobial activity, Gram positive bacteria, Gram negative bacteria, Microbial contamination, Storage.