Journal of Applied and Natural Science 4(2):297-303(2012)
In vitro regeneration of Momordica dioica (Roxb.)
Ashish R. Arekar, Janhavi A. Arekar, S. S. Barve and G. T. Paratkar
1Gokhale Education Society’s N. B. Mehta (Valwada) Science College, Department of Biotechnology, Bordi, Thane 401701 (MH), INDIA 2Kelkar Education Trust’s V. G. Vaze College of Arts, Science and Commerce, Department of Botany, Mithagar Road, Mulund (E), Mumbai 400081(MH), INDIA *Corresponding author. Email: email@example.com Received: July 19, 2012; Revised received: October 1, 2012; Accepted: November 20, 2012
Abstract : Momordica dioica, Roxb. (Family: Cucurbitaceae) commonly called as Kartoli, is an important medicinal plant, which has remained unexplored from the commercial point of view. Considering its scarce availability and the medicinal importance, in vitro cultures were established. Traditionally, M. dioica has been propagated mainly through its tuberous roots and less commonly by seeds. Germination through seeds is very difficult or impossible because of hard seed coat. As an alternative to traditional methods tissue culture offers an efficient method for propagation of M. dioica. Mature seeds were used for the regeneration of M. dioica. The decoated seeds of M. dioica were cultured on Murashige and Skoog basal medium (MS medium) supplemented with various combinations of Auxins (á – naphthaleneacetic acid) and Cytokinins (N6 - benzyl adenine). MS basal medium supplemented with 4.44 µM and 8.88 µM N6 - benzyl adenine (BA) gave rise to maximum number of shoots in 7-8 weeks. In vitro grown shoots were sub cultured on MS medium supplemented with different concentrations of indole-3-butyric acid (IBA) for root initiation. MS medium with 0.049mM indole-3-butyric acid (IBA) showed rooting in 45 days. The regenerated plantlets were successfully hardened in vermiculite.
Keywords : Hardening, Momordica dioica, Multiplication, Rooting, Seed Explants, Shoot Initiation