Journal of Applied and Natural Science 4(1):5-9(2012)
Micropropagation of Dendrocalamus strictus nees from mature nodal explants
B. N. Pandey* and N. B. Singh
Plant Physiology Laboratory, Department of Botany, University of Allahabad, Allahabad - 211 002, INDIA *Department of Botany, Government PG College, Lansdowne, Pauri Garhwal - 246 139, INDIA *Corresponding author. E-mail: firstname.lastname@example.org
Abstract : An effort has been made to propagate Dendrocalamus strictus Nees using nodal explants from mature plants. The explants, after surface sterilization, were inoculated on solidified MS basal medium with 2 mg/l BAP. The buds, which started growing, were transferred to solidified MS basal medium with 4 mg/l BAP and 15 mg/l AdS to achieve about three fold multiplication. Single shoots or 2-shoot clusters did not survive and therefore all the experiments were conducted with a cluster of minimum 3-shoots. The rooting was found difficult. Three hormones viz. IAA, IBA and NAA with a concentration of 1, 3 and 5 mg/l were used in various combinations for rooting. Out of the total 9 treatments, only 20% rooting was found only in one treatment, namely in the medium with 5 mg/l indole butyric acid (IBA).
Keywords : Bamboo, Dendrocalamus strictus, Micropropagation, Nodal explants