Journal of Applied and Natural Science 3(2):171-175(2011)
Evaluation of mutagenic potential of acetamiprid by dominant lethal test on Culex quinquefasciatus
Mamta Bansal 1 * and Asha Chaudhry 2
1Centre of Environment and Vocational Studies, Panjab University, Chandigarh-160014,India 2 Mosquito Cytogenetics Unit, Department of Zoology, Panjab University, Chandigarh-160014,India * Corresponding author. E-mail: email@example.com
Abstract : Acetamiprid, a member of the neonicotinoid insecticide family, is a new class of insecticide which has recently entered the market place. It is very selective and provides outstanding control of sucking pests such as aphids and whiteflies of major crops. In the present investigation, dominant lethal test (DLT) is adopted for the evaluation of the genotoxic effects of acetamiprid using Culex quinquefasciatus as an experimental model. In this experiment, the males hatched from larvae treated with LD 40 were cross mated with normal females and the results were based on the number of hatched and unhatched eggs laid by these females. The statistical analysis of the results for LD 40 treated groups gave the values of 37.526±3.886 as against 5.23±0.77 from the nontreated groups which indicated significant dominant lethality of p<0.01.These results indicated that exposure of pesticides even at small dose level proved deleterious to the genome of mosquito and its subsequent generation.
Keywords : Dominant lethality, Acetamiprid, Culex quinquefasciatus