Journal of Applied and Natural Science 3(1):75-77(2011)
Partial purification and characterisation of some low molecular weight α - amylases from Dolichos biflorus
Maryada Garg and Anoop K. Dobriyal*
Department of Zoology, H.N.B. Garhwal Central University Campus, Pauri Garhwal-246001, Uttarakhand, INDIA
*Corresponding author. E-mail: email@example.com
Abstract : Dolichos biflorus, a commonly used legume in Uttarakhand, produces alpha amylase enzyme for conversion of starch present in its cotyledons to glucose, so that this glucose can be further utilized for the life controlling processes, glycolysis and Kreb’s cycle. Yield of this α - amylase isolated from the germinating legume comes out to be 27.7 IUml -1 . Maximal amylase production occurs at pH 6.1 at 45O C. The enzyme was purified two fold, first with ultra-filtration and then with Ion-exchange chromatography. Ultra-filtration revealed size of amylase to be between 10 kDa and 30 kDa, against larger sizes of other bacterial amylases. The pH and temperature optima for purified enzyme were 6.1 and 45 OC respectively. The K m for starch came out to be 1.95 mgml-1 . This finding of generating one more new and low-price source of α - amylase is a great advancement in biotechnology.
Keywords : Ultra filtration, α - amylase, Yield, K m , V m