Journal of Applied and Natural Science 3(1):1-9(2011)
Transformation of 17 a -methyltestosterone in aquatic-sediment systems
Terence P. Barry* 1 , Padma Marwah 2,3 and Ashok Marwah* 2,3
1 Department of Animal Science, University of Wisconsin, 660 N Park St., Madison, WI 53706, USA
2 Department of Biochemistry-Institute for Enzyme Research, University of Wisconsin, 1710 University Avenue, Madison, WI 53726, USA.
3 Current address: Aptiance Inc., 5240 Tennyson Parkway, #207, Plano, TX 75024, USA
*Corresponding author.E-mail: firstname.lastname@example.org, email@example.com
Abstract : Two sediment samples that differed with respect to total organic carbon and texture (“sand” and “clay”) were exposed to radio inert 17 a -methyltestosterone (MT) or [ 14 C]-radiolabeled 17 a -methyltestosterone ( 14 C-MT), under both aerobic and anaerobic conditions, for up to 56 days, to characterize the fate of MT in the aquatic environment. Radio inert MT was quantified by a highly sensitive liquid chromatography/mass spectrometry (LC-MS) method and radioactive MT was quantified by HPLC using an in-line flow liquid scintillation counter (LSC). The data suggest that MT entering the aquatic environment is converted into metabolites that become tightly associated with the sediment. Half-lives for MT dissipation in the sediment systems ranged from 2-9 days, depending on the sediment type and the presence of oxygen. Sediment type had little effect on MT dissipation. The mineralization of MT under aerobic conditions was low (<9% conversion of MT to CO 2 ).
Keywords : 17 a -Methyltestosterone (MT), Aquatic-Sediment, Radio-HPLC, LC-MS, Aerobic, Anaerobic