Applied and Natural Science Foundation
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Journal of Applied and Natural Science
An International Journal | Print ISSN: 0974-9411 | Online ISSN: 2231-5209
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Journal of Applied and Natural Science 2(2):182-189(2010)
Development and validation of a High performance liquid chromatography-Mass spectrometry method for 17 a -methyltestosterone in Aquatic water systems
Ashok Marwah* 1, Xu Nianzu 2, Padma Marwah 1 and Terence P. Barry 3
1 Department of Biochemistry, Institute for Enzyme Research, University of Wisconsin at Madison, 1710 University Avenue, Madison, WI 53726, USA
3 Department of Animal Science, University of Wisconsin, 660 N Park St., Madison, WI 53706, USA
1 Current address: Chemilent Inc., 5240 Tennyson Parkway, #207 , Plano , TX 75024 , USA
2 Current address: Key Lab of Marine Biotechnology, Ningbo University , Ningbo , 513211, P. R. CHINA
*Corresponding author. E-mail:
Abstract : 17 a -Methyltestosterone is used as feed additive to manipulate the gender of fish for aquaculture. Earlier a simple, yet specific and robust validated high performance liquid chromatographic method has been developed for the determination of 17 a -methyltestosterone in fish feed. The present work describes a highly sensitive and robust Liquid Chromatography-Mass Spectrometry method for the quantitation of 17 a -methyltestosterone in aquatic water systems using 17 β -hydroxy-3 β -methoxyandrost-5-en-7-one as internal standard. The method was validated in the concentration range of 0.2 to 25 ng of 17 a -methyltestosterone on column leading to a limit of quantitation of 0.08 ppb or 0.08 mg/L in water, and has potential to increase the limit of detection and quantitation by an order of magnitude, if required.

Keywords : High-performance liquid chromatography-Mass spectrometry, Electrospray ionization, 17 a -Methyltestosterone, Solid phase extraction, Method validation
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