Applied and Natural Science Foundation
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Journal of Applied and Natural Science
An International Journal | Print ISSN: 0974-9411 | Online ISSN: 2231-5209
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Journal of Applied and Natural Science 2(1):88-92(2010)
Characterization and evaluation of heavy metal tolerance of bacterial species from soil of waste area near Riyan steel rolling mills, Muzaffarnagar, India
Satyajeet Khare*1 Niaz Ahmed1, Shailja Pant1 and RamDas2
1Department of Microbiology, Dolphin PG Institute of Biomedical and Natural Sciences, Manduwala, Dehradun-247008 (Uttrakhand), INDIA
2National JALMA Institute for Leprosy & Other Mycobacterial Disease, Tajganj, Agra (U.P.), INDIA
*Corresponding author. E-mail:
Abstract : The present study observed a variety of microorganisms from the soil of the waste area near Riyan Steel Rolling Mills , Muzaffarnagar. Among the microorganisms, 10 isolates of Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus showed great potential against detrimental heavy metals like Hg, Pb, Zn. Out of these 10 isolates, only 2 isolates of Bacillus subtilis (N1A2, N1P2), one isolates of P. aeruginosa N1A4 and one isolates of S. aureus A1N3 showed tremendous tolerance against various heavy metals. Bacillus subtilis N1A2 showed 15x109 cfu/ml in 100µg/ml of HgCl2 whereas B. subtilis N1P2 showed 15x109 cfu/ml in 400µg/ml of zinc acetatate. Similarily Pseudomonas aeruginosa N1A4 showed 11x109 cfu/ml in 150µg/ml of lead acetate. Staphylococcus aureus A1N3 showed great resistance towards 450µg/ml of zinc sulphate and maintain their growth up to 7x109 cfu/ml. strains were identified as Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus on the basis of their morphological, physiological and biochemical and compared with their standard MTCC strains.

Keywords : Heavy metals, CFU, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus
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