Journal of Applied and Natural Science 1(2):269-274(2009)
RAPD-PCR based genomic characterization of two populations of Culex quinquefasciatus (Diptera : Culicidae)
T. Kaura, P. Kaushal, M. Bansal and S. Chaudhry*
Mosquito Cytogenetics Unit, Department of Zoology, PanjabUniversity, Chandigarh-160014, INDIA,
* Corresponding author. E-mail: firstname.lastname@example.org
Abstract : The present paper deals with the RAPD-PCR based genomic characterization of Culex quinquefasciatus Say which is a major vector of filariasis in several parts of the Indian subcontinent. One population of the test organism used in the study was procured from Goa (pop.A) while the other (pop.B) was collected from a village Nadasahib (20 kms from Chandigarh). The RAPD-PCR amplification of whole body homogenate of freshly hatched individual specimens was carried out by using three random primers: primer I- 5’- GTCCCGACGA – 3’; primer II- 5’ – TGATCCCTGG – 3’ and primer III- 5’- GTGACGTAGG – 3’. Primer Iproduced 5 distinct bands from the DNA of pop. A, whose base composition ranged from 200-1000 bp. Likewise, 7 bands ranging from 130-750 bp and 4 bands ranging from 270-950 bp were generated with primers II and III respectively. In case of pop.B, a total of 8 bands ranging from 200-1000 bp were generated with primer I. Similarly, a total of 6 bands ranging from 250-900 bp with primer II and 4 bands ranging from 180-950 bp with primer III were produced. Based on the band sharing coefficient and the application of Nearest Neighbour Joining (NJ) analysis it was found that primer I was more suitable for detecting genomic differences at the species and generic levels while primer II was ideal for detecting variations in the number of bp in RAPD generated bands among different populations of Cx. Quinquefasciatus.
Keywords : PCR, Cx. quinquefasciatus, Genomics